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Biochemistry - Laboratory Techniques - MCAT Practice Questions
Gel electrophoresis separates charged molecules primarily by which two
properties?
Size and charge
In gel electrophoresis, which molecules move faster through the gel?
Small molecules
Which type of gel has large pores and is suitable for separating nucleic
acids?
Agarose gel
Which type of gel has small pores and is better for separating proteins?
Polyacrylamide gel
What does SDS (Sodium dodecyl sulfate) do to proteins in SDS-PAGE?
Denatures proteins and gives them uniform negative charge
SDS-PAGE separates proteins based on which property?
Mass only
Native-PAGE separates proteins based on which properties?
Both size and charge
In isoelectric focusing, proteins stop migrating when they reach which
condition?
pH that matches their isoelectric point (pI)
Which blotting technique is used to detect specific DNA sequences?
Southern blotting
Which blotting technique is used to detect specific RNA sequences?
Northern blotting
Which blotting technique is used to detect specific proteins?
Western blotting
What is the primary purpose of PCR (Polymerase Chain Reaction)?
Amplify specific target regions of DNA
What type of DNA polymerase is used in PCR?
Taq polymerase (thermostable)
What are the three steps of a PCR cycle in order?
Denaturation, Annealing, Extension
Sanger sequencing is based on the incorporation of which molecules?
Chain-terminating dideoxynucleotides