Biochemistry - Glycolysis - MCAT Practice Questions
Glycolysis breaks down one molecule of glucose into how many molecules of
pyruvate?
Two molecules
What is the net ATP gain from glycolysis?
2 ATP
How many ATP molecules are consumed during the investment phase of
glycolysis?
2 ATP
How many ATP molecules are produced during the payoff phase of
glycolysis?
4 ATP
How many NADH molecules are produced in glycolysis?
2 NADH
Which enzyme catalyzes the first ATP-consuming step of glycolysis?
Hexokinase
Which enzyme catalyzes the second ATP-consuming step and is a major
regulatory point in glycolysis?
Phosphofructokinase (PFK)
Which enzyme catalyzes the final ATP-producing step of glycolysis?
Pyruvate kinase
Which enzyme requires inorganic phosphate (Pi) and NAD⁺ and produces NADH in
glycolysis?
Glyceraldehyde 3-phosphate dehydrogenase
Which enzyme produces water (H₂O) as a byproduct during glycolysis?
Enolase
Glucose is a molecule with how many carbon atoms?
6 carbons
Pyruvate is a molecule with how many carbon atoms?
3 carbons
Which enzyme catalyzes an ATP-producing step by converting ADP to ATP in the
middle of glycolysis?
Phosphoglycerate kinase
Which of the following is NOT one of the three major regulatory enzymes in
glycolysis?
Aldolase
How many total steps are involved in the glycolytic pathway?
10 steps